Sir William CHAMBERLAYNE
1485 - 
| Francis CHAMBERLAIN 1589-1623 |
| Francis CHAMBERLAIN | |
| B: | 1589 |
| Billericay, Essex, England | |
| D: | 1623 |
| Boston, Suffolk, MA | |
 | Agnes HAIDEN 1591-1621 |
| Agnes HAIDEN | |
| B: | 1591 |
| Billerica, Essex, England | |
| M: | 1613 |
| Essex, England | |
| D: | 1621 |
| Billerica, Middlesex Co., MA | |
| Thomas CHAMBERLAIN 1564-1633 |
| Thomas CHAMBERLAIN | |
| B: | 1564 |
| Great Burstead, Essex, England | |
| D: | 1633 |
| Bradwell, Essex, England | |
| Dorothie JACKSON 1564-1633 |
| Dorothie JACKSON | |
| B: | 1564 |
| Great Burstead, Essex, England | |
| M: | 22 Nov 1589 |
| Great Burstead | |
| D: | 1633 |
| Yorkshire, England | |
| Sir Thomas CHAMBERLAIN 1522-1580 |
| Sir Thomas CHAMBERLAIN | |
| B: | 1522 |
| Presbury, Gloucester, England | |
| D: | 26 Jul 1580 |
| Gloucester, Gloucestershire, England | |
| Elizabeth Jane LUDDINGTON 1530-1580 |
| Elizabeth Jane LUDDINGTON | |
| B: | 1530 |
| M: | 1558 |
| Presbury, Gloucester, England | |
| D: | 1580 |
| Sir William CHAMBERLAYNE 1485- |
| Sir William CHAMBERLAYNE | |
| B: | 1485 |
| Presbury, Gloucester, England | |
| D: | Glouchestershire, England |
| Elizabeth FLEMING 1488-1522 |
| Elizabeth FLEMING | |
| B: | 1488 |
| M: | 1522 |
| D: | 1522 |