Family: Sir Thomas CHAMBERLAIN / Elizabeth Jane LUDDINGTON (F256)
m. 1558
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 | Sir William CHAMBERLAYNE (1485- ) |
 | Elizabeth FLEMING (1488-1522) |
| Sir Thomas CHAMBERLAIN (1522-1580)
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| Elizabeth Jane LUDDINGTON (1530-1580) |
| Thomas CHAMBERLAIN (1564-1633)  |